Titlebook: RNA Therapeutics; Function, Design, an Mouldy Sioud Book 2010 Humana Press 2010 DNA.Microarray.PCR.Transplantat.Tumor.cloning.gene expressi

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In Situ Hybridization Detection of microRNAs,scribe a protocol for miRNA in situ hybridization on mouse tissue cryosections using locked nucleic acid (LNA) probes. LNA probes demonstrate a much higher affinity to their complimentary RNAs compared to conventional RNA and DNA oligo probes, which allow detection of miRNAs in tissue sections with excellent specificity.

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1064-3745 rapeutic proteins.Features the translation of RNA-based ther.Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutic

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Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interfereduced the number of unwanted off-target genes without interfering with siRNA silencing potency of the anticipated target gene. This chapter describes how to separate gene silencing from immunostimulation. Also, it discusses the impact of these findings on the design of effective cancer vaccines.

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Quantitative Analysis of RNA Modifications,. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of techniques, including oligonucleotide-affinity selection, site-specific cleavage and radiolabeling, nuclease digestion, and thin layer chromatography.

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Systemic Delivery of Synthetic siRNAs,llenge have been described, including cationic lipids, high-pressure injection, viral vectors, and chemical modifications of the siRNAs. This chapter describes cationic lipid delivery of siRNAs to adult mice.

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Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migratio cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.

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RNA Modifications: A Mechanism that Modulates Gene Expression,l modifications, including 2?-O-methylation and pseudouridylation, which are functionally important. In this chapter, we highlight the functional aspects of the modifications of spliceosomal snRNA and rRNA and provide a framework for understanding how posttranscriptional modifications are capable of influencing gene expression.