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Titlebook: RNA Silencing; Methods and Protocol Gordon G. Carmichael Book 2005 Humana Press 2005 DNA.Vivo.gene expression.genes.molecular biology.regul

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書目名稱RNA Silencing
副標(biāo)題Methods and Protocol
編輯Gordon G. Carmichael
視頻videohttp://file.papertrans.cn/821/820193/820193.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: RNA Silencing; Methods and Protocol Gordon G. Carmichael Book 2005 Humana Press 2005 DNA.Vivo.gene expression.genes.molecular biology.regul
描述A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology? series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
出版日期Book 2005
關(guān)鍵詞DNA; Vivo; gene expression; genes; molecular biology; regulation; transcription
版次1
doihttps://doi.org/10.1385/1592599354
isbn_softcover978-1-61737-568-2
isbn_ebook978-1-59259-935-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2005
The information of publication is updating

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siRNA Delivery In Vivo,RNAs) (.–.). This is now driven predominantly by siRNAs, as they are not technically demanding as traditional antisense and ribozyme technologies (.,.). The success of siRNAs as therapeutics, however, is largely dependent on the development of a delivery vehicle that can efficiently deliver them in vivo.
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Nathaniel R. Dudley,Bob Goldstein (.MLCT) excited state is lowest in energy. The pronounced solvatochromic and rigidochromic effects of the Ir. compounds are responsible for the reversal of the order of the lowest excited states. Mixing between the π-π* and MLCT excited states is reflected in the oscillator strengths, luminescence
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