Titlebook: RNA Scaffolds; Methods and Protocol Luc Ponchon Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2021

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Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging,ly used for the cellular imaging, but the choice of targets is still limited. Recently, fluorogenic RNA-based sensors gained lots of attention. This novel sensor system can function as a general platform for various cellular targets. Here, we describe the steps to rationally design, optimize, and ap

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,FRET Analysis of RNA –Protein Interactions Using Spinach Aptamers,ery of fluorescent light-up aptamers (FLAPs), short RNA aptamers that switch the fluorescence of small, cell-permeable, and nontoxic organic chromophores on, paved the road for their utilization in direct RNA–protein interactions. The combination with fluorescent proteins as biological fluorophores

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Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vi aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofa

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Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells,itch libraries are constructed by randomizing the key nucleotides that couple the molecular recognition function of an aptamer with the self-cleavage activity of a ribozyme. The allosteric ribozyme (aptazyme) candidates are cloned in the 3′ untranslated region (UTR) of a reporter gene mRNA. The plas

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,Folding RNA–Protein Complex into Designed Nanostructures,otifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nanoobjects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we desc

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