Titlebook: RNA Methylation; Methods and Protocol Alexandra Lusser Book 2017 Springer Science+Business Media LLC 2017 posttranscriptional base modifica

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書(shū)目名稱(chēng)RNA Methylation影響因子(影響力)




書(shū)目名稱(chēng)RNA Methylation影響因子(影響力)學(xué)科排名




書(shū)目名稱(chēng)RNA Methylation網(wǎng)絡(luò)公開(kāi)度




書(shū)目名稱(chēng)RNA Methylation網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書(shū)目名稱(chēng)RNA Methylation被引頻次




書(shū)目名稱(chēng)RNA Methylation被引頻次學(xué)科排名




書(shū)目名稱(chēng)RNA Methylation年度引用




書(shū)目名稱(chēng)RNA Methylation年度引用學(xué)科排名




書(shū)目名稱(chēng)RNA Methylation讀者反饋




書(shū)目名稱(chēng)RNA Methylation讀者反饋學(xué)科排名

Type-1-Diabetes

Cytokines

Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation determination of modified nucleosides in both DNA and RNA. Here, we describe a protocol to analyze m.A in RNA by LC-ESI-MS/MS. And this protocol also can be extended to the analysis of other modified nucleosides in both DNA and RNA.

muffler

Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq).A positive RNA fragments are subsequently sequenced by RNA-seq in parallel with background control non-immunoprecipitated input RNA fragments. Analyses reveal peaks of m.A enrichment containing sites of modifications analogous to chromatin modification immunoprecipitation experiments.

fender

Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencingissues. Although the nature of the bisulfite sequencing protocol makes it comparably easy to translate from a low to a high-throughput approach, several critical points require attention before starting such a project. We describe a step-by-step protocol for planning and performing the experiment and analyzing the data.

manifestation

Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP) we describe how to use these mutational signatures to map m6A residues at nucleotide resolution. Taken together, our protocol allows for high-throughput detection of individual m6A residues throughout the transcriptome.

Gratulate

Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)thyl-6-adenosine (m6A) modification at nucleotide resolution in the human transcriptome. Here we describe the m5C-miCLIP protocol, discuss how it yields the nucleotide-resolution RNA modification maps, and comment on how these have contributed to the new field of molecular genetics research coined “epitranscriptomics.”

愛(ài)好

Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencingysis of cytosine methylation in low abundance poly(A)RNA using a combination of commercially available kits and standard lab methods to ensure reproducible results. Furthermore, useful information on optimizing the method, suitable controls for almost all steps, and general troubleshooting guides are provided.

dysphagia

Book 2017focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-

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Detection and Quantification of ,,-Methyladenosine in Messenger RNA by TLCs often useful to directly measure and compare ..-methyladenosine levels between samples. Two dimensional chromatography of radiolabeled nucleotides, following specific nuclease treatments, provides a robust, sensitive, and reproducible assay for this modification.