Titlebook: RNA Mapping; Methods and Protocol M. Lucrecia Alvarez,Mahtab Nourbakhsh Book 2014 Springer Science+Business Media New York 2014 RNA boundar

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Analysis of RNA Secondary Structureanscription process, the partially synthesized RNA is folded by base-pairing and thermodynamic intramolecular or intermolecular interactions. This results in a dynamic spreading of a secondary structure along the length of the transcribed section of the RNA. The analysis of both primary or secondary

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Tertiary Structure Mapping of the Pri-miRNA miR-17~92 gene regulation beyond spliceosomal, ribosomal, and messenger RNAs. Approximately one half of miRNAs are polycistronic, where two or more miRNAs are encoded on a single pri-miRNA transcript, termed a miRNA cluster. The six miRNAs of the miR-17~92 cluster are contained within a ~800 nucleotide regio

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Quantification of miRNAs by a Simple and Specific qPCR Methodn disease. The rapid pace of the emerging field of miRNAs has opened new avenues for development of techniques to quantitatively determine miRNA expression levels in different systems..In this chapter we describe a PCR method for quantification of miRNAs based on a single reverse transcription react

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Sequencing Small RNA: Introduction and Data Analysis FundamentalsNAs seems to be an obvious choice to understand their expression and its downstream effect. Additionally, sequencing provides an opportunity to identify novel and polymorphic miRNA. However, the biggest challenge is the appropriate data analysis pipeline, which is still in phase of active developmen

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Measuring Expression Levels of Small Regulatory RNA Molecules from Body Fluids and Formalin-Fixed, Pods enable us to monitor their presence in various types of samples but we need flexible methods for validating datasets generated by high-throughput methods..Here we describe the detailed protocols to be used with our MiRNA Primer Design Tool assay design system. The presented methods allow the fle

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