Titlebook: RNA Folding; Methods and Protocol Christina Waldsich Book 2014 Springer Science+Business Media, LLC 2014 Folding pathway dissection.In vivo

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The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis,ndard protein enzymes, for which reactions can usually be started by mixing the enzyme with its substrate, ribozymes are typically self-cleaving. The reaction has to be initiated by folding the RNA into its active conformation. Thus, ribozyme kinetics are influenced by both folding and catalytic com

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RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding,RNA chaperone proteins on the folding rate. The crux of this approach is that only the natively folded RNA is able to perform the catalytic reaction. This method can provide a quantitative measure of the fraction of native RNA over time, and it can readily distinguish the native state from all misfo

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RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis,formation about such native structures in solution as a function of experimental conditions. The application of native gel electrophoresis in a comparative manner allows to obtain precise information on relative angles subtended between given pair of stems in an RNA molecule. By adapting this approa

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Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation,r interactions in solution. When operated in a sedimentation velocity (SV) recording mode, it reports precisely on the hydrodynamic properties of a molecule, including its sedimentation and diffusion coefficients, which can be used to calculate its hydrated radius, as well as, to estimate its global

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RNA Folding Dynamics Using Laser-Assisted Single-Molecule Refolding,ys is critical to understanding how RNA molecules acquire their biological function. We have previously developed a novel approach to help characterize such misfolded intermediates. Laser-assisted single-molecule refolding (LASR) is a powerful technique that combines temperature-jump (T-jump) kineti

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Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Smallopy (FCS) and small angle X-ray scattering (SAXS) measurements can be used to determine intermolecular interactions between RNA and its binding partners. To define oligomerization states of free RNA and its complexes with bound target molecules, hydrodynamic radii, radii of gyration as well as the m

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1064-3745 ential for reproducible results.Contains key notes and vital.Focusing on in vitro and intracellular RNA structure formation, .RNA Folding: Methods and Protocols. provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the s