Titlebook: RNA Detection; Methods and Protocol Imre Gaspar Book 2018 Springer Science+Business Media LLC 2018 Drosophila.ECHO-liveFISH.FISH.RCas9.FISS

Heretical

乞丐

無(wú)所不知

腐敗

Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryafish embryos, an smFISH protocol optimized for zebrafish cryosections, and a user-friendly, automated analysis pipeline for cell segmentation and transcript detection. The software is freely available and can be used to analyze sections of any multicellular organism.

機(jī)制

In Situ Detection of MicroRNA Expression with RNAscope Probes, provides simultaneous single molecule detection of individual microRNA and its target gene. This method allows for rapid and sensitive detection of noncoding RNA transcripts in frozen tissue sections.

憤慨一下

Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH, entangled experimental results. Using this method, we observe in living brain tissue spatially constrained nuclear RNA foci under dynamic regulation in response to cellular transcriptional activity with individual cell heterogeneity.

膽大

Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers,orophores and also dual-color imaging of two distinct RNAs in live bacteria. Our approach uses small RNA tags and small molecule fluorophores for labeling, thereby minimal perturbation on the function and dynamics of the RNA of interest is expected.

AMOR

Neonatal

wangle