Titlebook: RNA; Methods and Protocol Henrik Nielsen Book 2011 Springer Science+Business Media, LLC 2011 Bioinformatics.Mammalian genome.Messenger RNAs

Wilson

書目名稱RNA影響因子(影響力)




書目名稱RNA影響因子(影響力)學科排名




書目名稱RNA網(wǎng)絡公開度




書目名稱RNA網(wǎng)絡公開度學科排名




書目名稱RNA被引頻次




書目名稱RNA被引頻次學科排名




書目名稱RNA年度引用




書目名稱RNA年度引用學科排名




書目名稱RNA讀者反饋




書目名稱RNA讀者反饋學科排名

Initial

Ambiguous

Working with RNAk rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modificatio

antidepressant

Synthesis of RNA by In Vitro Transcriptionto those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts

遺傳

Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capturepoly(A). RNA directly from guanidinium thiocyanate (GuSCN)-containing cell or tissue extract by combining the design of biotinylated LNA oligo(T) capture probes with subsequent immobilization of the captured poly(A). RNA onto streptavidin-coated magnetic particles. In contrast to DNA oligo-dT and po

ATRIA

Axillary

muscle-fibers

interior

Rapid Amplification of cDNA Ends (RACE)ly known. Starting with an mRNA mixture, gene-specific primers generated from the known regions of the gene and non-specific anchors, full-length sequences can be identified in as little as 3 days. RACE can also be used to identify alternative transcripts of a gene when the partial or complete seque

整頓

Fractionation of mRNA Based on the Length of the Poly(A) Taile mRNAs according to the length of the poly(A) tail. Poly(A) fractionation can be used to detect small changes in poly(A) tail length or to prepare samples for microarray analysis. RNA or crude lysate is mixed with biotinylated oligo(dT), which is then bound to paramagnetic streptavidin beads. Oligo