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Titlebook: R-Loops; Methods and Protocol Andrés Aguilera,Alexey Ruzov Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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發(fā)表于 2025-3-21 18:24:24 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)R-Loops
副標(biāo)題Methods and Protocol
編輯Andrés Aguilera,Alexey Ruzov
視頻videohttp://file.papertrans.cn/821/820035/820035.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: R-Loops; Methods and Protocol Andrés Aguilera,Alexey Ruzov Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen
描述This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids .in vitro. and .in vivo.. Several protocols permit studying non-canonical RNA nucleotides in the R-loop context, as well as a number of specific protocols devoted to the investigation of R-loop topology and their functional roles in the biology of mitochondria and telomeres. Finally, a large block of chapters is dedicated to different methods allowing genome-wide mapping of DNA-RNA hybrids in various organisms. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .R-Loops: Methods and Protocols. serves as an ideal resource for those working on R-loop ho
出版日期Book 2022
關(guān)鍵詞DNA-RNA hybrids; Genomic stress and instability; Genome dynamics; Electron microscopy; Topology; Genome-w
版次1
doihttps://doi.org/10.1007/978-1-0716-2477-7
isbn_softcover978-1-0716-2479-1
isbn_ebook978-1-0716-2477-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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發(fā)表于 2025-3-21 21:54:38 | 只看該作者
https://doi.org/10.1007/978-1-0716-2477-7DNA-RNA hybrids; Genomic stress and instability; Genome dynamics; Electron microscopy; Topology; Genome-w
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發(fā)表于 2025-3-22 02:50:05 | 只看該作者
978-1-0716-2479-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Highly Purified Top1-Bound DNA Fragments,in, CPT) in living cells. Various methods are available to detect Top1ccs in cultured cells, including protocols based on the use of specific antibodies. Here, we describe a protocol to isolate Top1ccs at high purity, which does not depend on antibodies.
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發(fā)表于 2025-3-22 19:42:10 | 只看該作者
Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids,m.C) marked RNA:DNA hybrids containing the m.C modified RNA strand add another layer of regulation to the R-loop structure. Here we describe the detection of m.C modifications in the context of RNA:DNA hybrids using immunostaining, and identification of specific binding partners of m.C marked RNA:DNA hybrids using pull-down method.
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Henriette Stoy,Joel Luethi,Fabienne K. Roessler,Johannes Riemann,Andres Kaech,Massimo Lopesf similarity, e.g. in a closed hemispherical container, also led to serious difficulties. Consequently, the cause of difficulties should be sought in the physical statement of the problem, which probably does not take into account an essential mechanism limiting the growth of velocities in the flow
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