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Titlebook: Quantitation of mRNA by Polymerase Chain Reaction; Nonradioactive PCR M Thomas K?hler,Dirk La?ner,Barbara Pustowoit Book 1995 Springer-Verl

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書目名稱Quantitation of mRNA by Polymerase Chain Reaction
副標(biāo)題Nonradioactive PCR M
編輯Thomas K?hler,Dirk La?ner,Barbara Pustowoit
視頻videohttp://file.papertrans.cn/781/780780/780780.mp4
叢書名稱Springer Lab Manuals
圖書封面Titlebook: Quantitation of mRNA by Polymerase Chain Reaction; Nonradioactive PCR M Thomas K?hler,Dirk La?ner,Barbara Pustowoit Book 1995 Springer-Verl
描述In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
出版日期Book 1995
關(guān)鍵詞Analyse; DNA; DNA sequencing; Nucleotide; PCR; Quantitative PCR; RNA; chemical analysis; chemische Analyse; e
版次1
doihttps://doi.org/10.1007/978-3-642-79712-5
isbn_softcover978-3-642-79714-9
isbn_ebook978-3-642-79712-5
copyrightSpringer-Verlag Berlin Heidelberg 1995
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