| 書目名稱 | Protein Targeting Protocols | | 編輯 | Mark Giezen | | 視頻video | http://file.papertrans.cn/762/761780/761780.mp4 | | 概述 | Thorough and state-of-the-art update of the successful first edition.Emphasis placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.Targeting protocols from a | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems...Authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into | | 出版日期 | Book 2007Latest edition | | 關鍵詞 | Escherichia coli; Organelle; Polyacrylamid-Gelelektrophorese; bioinformatics; electron microscopy; genes; | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-59745-466-7 | | isbn_softcover | 978-1-61737-727-3 | | isbn_ebook | 978-1-59745-466-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2007 |
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