| 書目名稱 | Protein Engineering Protocols | | 編輯 | Katja M. Arndt,Kristian M. Müller | | 視頻video | http://file.papertrans.cn/762/761669/761669.mp4 | | 概述 | Comprehensive guide.a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry.New methods for DNA shuffling.Methods in Molecular Biology? series format.Incl | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generatio | | 出版日期 | Book 2007 | | 關鍵詞 | Activation; Amino acid; Calcium; DNA; Elongation; Nucleotide; biochemistry; enzymes; escherichia coli; genes; | | 版次 | 1 | | doi | https://doi.org/10.1385/1597451878 | | isbn_softcover | 978-1-61737-338-1 | | isbn_ebook | 978-1-59745-187-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2007 |
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