| 書(shū)目名稱(chēng) | Principles and Technical Aspects of PCR Amplification | | 編輯 | Elizabeth Pelt-Verkuil,Alex Belkum,John P. Hays | | 視頻video | http://file.papertrans.cn/756/755430/755430.mp4 | | 概述 | Gives a broad overview of available PCR techniques and variants.Focussing on the PCR technique, not restricted to a few limited applications associated with a minor discipline.Chapters outlining appro | | 圖書(shū)封面 |  | | 描述 | .Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/absence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes. . . .Frequently, PCR technicians and specialists limit their understanding of PCR to one particular methodology. However, this approach limits their appreciation of the range of versatile PCR techniques currently available, techniques that may be applicable and indeed more suitable to their own laboratory situation.. . .This manual aims to provide thereader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many im | | 出版日期 | Book 2008 | | 關(guān)鍵詞 | DNA; Nucleotide; PCR; Quantitative PCR; Vivo; methodology | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4020-6241-4 | | isbn_softcover | 978-90-481-7579-6 | | isbn_ebook | 978-1-4020-6241-4 | | copyright | Springer Science+Business Media B.V. 2008 |
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