| 書(shū)目名稱 | Photoswitching Proteins | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Dominik Niopek | | 視頻video | http://file.papertrans.cn/747/746689/746689.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書(shū)名稱 | Methods in Molecular Biology | | 圖書(shū)封面 |  | | 描述 | This volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions | | 出版日期 | Book 2020 | | 關(guān)鍵詞 | Optogenetic manipulation; electrophysiology; transgene regulation; light-oxygen-voltage domains; nucleoc | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-0755-8 | | isbn_softcover | 978-1-0716-0757-2 | | isbn_ebook | 978-1-0716-0755-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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