Titlebook: Parasite Genomics Protocols; Christopher Peacock Book 2015Latest edition Springer Science+Business Media New York 2015 epigenome.genetic v

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Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in ,,ack control at the level of transcription initiation. A large number of . transcripts harbor in their 3′UTRs two phylogenetically distinct subfamilies of extinct Short Interspersed DEgenerate Retroposons (SIDER1 and SIDER2) that are involved in posttranscriptional regulation of gene expression. We h

PATRI

Gene Suppression in Schistosomes Using RNAi,ar parasitic platyhelminths called schistosomes. Treatment and control of schistosomiasis rely on a single drug, praziquantel, and concern exists over the possible emergence of resistance to this drug. The recent completion of the genome sequences of the three main worm species that cause schistosom

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jarring

The Genome-Wide Identification of Promoter Regions in ,,r mechanisms that underlie transcriptional regulation, it is essential to identify the exact positions of the transcriptional start sites (TSSs) and characterize the upstream promoter regions. However, it has been essentially impossible to obtain comprehensive information using conventional methods.

saturated-fat

RNA-Seq Approaches for Determining mRNA Abundance in ,,pping the transcripts to the nucleotide level. Here we describe an RNA-seq approach that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5′ end of all . (and other trypanosomatid) mRNAs.