Titlebook: Optogenetics; Methods and Protocol Arash Kianianmomeni Book 2016 Springer Science+Business Media New York 2016 algal sensory photoreceptors

Ovulation

Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats,ty modulation on free-behaving rats using optogenetic techniques. The target sites of the rat brain were dorsal periaqueductal gray (dPAG) and ventral tegmental area (VTA) for the modulation of defensive and reward behaviors, respectively.

演講

Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for light stimulation to brain. Here, we describe a two-part methodology starting with a procedure to inject an optogenetic AAV virus into rat cortex followed by a second procedure to surgically implant an optical cannula for light delivery to the deeper cortical layers.

interlude

Optogenetic Tools for Confined Stimulation in Deep Brain Structures,cattering in biological tissues make it difficult to illuminate a minute volume, especially in the deep brain, without the use of optical fibers to guide light. This work describes the design and the in vivo application of a side-firing optical fiber adequate for delivering light to specific regions within a brain subcortical structure.

senile-dementia

壓倒性勝利

An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in ortant. We monitored transfected cell health by measuring electrophysiological parameters, and used them as a guideline to optimize transfection. Here we describe an optimized transfection protocol that achieves reasonably high efficiency (10–20 %) with no discernable impact on cell health, as characterized by electrophysiology.

聲明

1064-3745 ation advice from the experts.Includes supplementary materia.This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications. .Optogenetics:Methods and Protocols. contains a collection of recently developed technicalprotocols on optogen

無(wú)節(jié)奏

Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches, millisecond and micrometer precision. In this chapter, we describe the engineering of light-activated and -inhibited neuronal nicotinic acetylcholine receptors, as well as their characterization and use in cultured cells.

VEN

Combined Optogenetic and Chemogenetic Control of Neurons,cule substrate. Such luminescent opsins, luminopsins, allow conventional fiber optic use of optogenetic sensors, while at the same time providing chemogenetic access to the same sensors. We describe applications of this approach in cultured neurons in vitro, in brain slices ex vivo, and in awake and anesthetized animals in vivo.

Visual-Field

Book 2016s and Protocols. contains a collection of recently developed technicalprotocols on optogenetic applications in neuroscience, brain mapping, treatmentof neurological disorders, and restoration of visual function. Severalintroductory and discussion chapters offer a wide overview about sources anddiver

leniency