Titlebook: Opioid Receptors; Methods and Protocol Santi M. Spampinato Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer N

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painkillers

Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissuest restricted to the central nervous system, but are also present in peripheral sensory neurons, where their activation exerts analgesic effects without inducing centrally mediated side effects. Here, we describe immunohistochemical analysis of μ-opioid receptors in the peripheral sensory neuron cell

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Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Micecurs has not been completely elucidated to date. One possible hypothesis involves the internalization of MOR. Here, we describe a simple in vitro protocol to investigate the localization of MOR-1 after repeated morphine administration in the spinal cord of morphine-tolerant mice, using western blott

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GTPγS-Autoradiography for Studies of Opioid Receptor Functionality70s and several drugs and peptides have emerged over the years. In 2012, the crystal structures were also demonstrated, with new data on the receptor sites, and thus new possibilities will appear. The role of opioids in the brain has attracted considerable interest in several diseases, especially pa

Agnosia

Evaluating Opioid-Mediated Adenylyl Cyclase Inhibition in Live Cells Using a BRET-Based Assay. Here, we describe a simple and cost-effective method of measuring adenylyl cyclase inhibition, one of the hallmarks of opioid receptor activation. The assay is based on bioluminescence resonance energy transfer (BRET) that involves transfection of a biosensor in human embryonic kidney (HEK)-293 ce

Pseudoephedrine

Patch?Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nd description of patch?clamp protocol to measure the effect of a μ-opioid receptor agonist on the activity of G?protein-coupled inwardly rectifying potassium (GIRK or Kir3) channels. This is performed in peripheral sensory neurons isolated from dorsal root ganglia (DRG) of mice without or with a chr

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