Titlebook: Oogenesis; Methods and Protocol Ioannis P. Nezis Book 2016 Springer Science+Business Media New York 2016 plants.oogenesis.mosquitos.chicken

antecedence

Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting,of the proteins Ddx4 or Ifitm3, alone or in combination with the use of fluorescent reporter mice. Beginning with sample preparation, we provide point-by-point instructions to guide researchers on how to isolate OSCs using flow cytometry.

小蟲(chóng)

彩色

Immuno-Gold Labeling of , Follicles for Transmission Electron Microscopy,ples, which focus at the retention of antigenicity in efforts to mirror the native state of the tissues and cells as closely as possible. Herein, we describe the methodology for immuno-gold labeling of . follicles, following preparation of the samples using the Tokuyasu method for ultracryosectioning.

FRET

災(zāi)禍

小平面

Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes,oinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method with approaches for the perturbation of specific proteins opens up opportunities for understanding the molecular and intracellular basis of mammalian meiosis.

GUMP

Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes,re we describe several reliable methods for microtubule and/or microfilaments preservation and visualization in . oocyte extracts, and in situ in live and fixed insect and frog (.) oocytes. In addition, we describe visualization of cytoskeleton-bound RNAs using molecular beacons in live . oocytes.

全國(guó)性

Book 2016pective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls...Authoritative and cutting-edge, .Oogenesis:Methods and Protocols. aims to ensure successful results in the furtherstudy of this vital field..

PAGAN

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