Titlebook: Odontogenesis; Methods and Protocol Chrissa Kioussi Book 2012 Springer Science+Business Media, LLC 2012 Bioengineered teeth.Developmental m

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Book 2012aiting for a full understanding of the developmental pathways at the molecular level.? Written in the highly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily repro

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Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tissh development and dissected bony parts. The description of these protocols is followed by the outline of a quick decalcification method and the procedure for embedding in epoxy resin to obtain serial sections with high histological quality.

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1064-3745 tep-by-step protocols, ready for the lab.Features tips from .Only in recent times has the possibility of growing and implanting replacement teeth, made from one’s own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be s

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Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Geioengineered tooth in vivo. This method is expected to be utilized as a valuable technique for analyzing gene and protein functions during organogenesis. Here, we describe protocols for tooth germ reconstitution using the organ germ method and methods for analyzing tooth development in vitro and in vivo.

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Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts,k the translation of mRNA to proteins in order to study specific gene functions and identify target molecules. Importantly, siRNAs have the potential to be used in treatment of disease. Here, we describe how the siRNA technology can be used to knock down genes in dental tissue-derived cells using integrin α11 knockdown as an example.

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