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Titlebook: Nucleic Acid Hybridization in the Study of Cell Differentiation; H. Ursprung Book 1972 Springer-Verlag Berlin Heidelberg 1972 Cell.Nuklein

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發(fā)表于 2025-3-21 16:58:33 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Nucleic Acid Hybridization in the Study of Cell Differentiation
編輯H. Ursprung
視頻videohttp://file.papertrans.cn/669/668776/668776.mp4
叢書名稱Results and Problems in Cell Differentiation
圖書封面Titlebook: Nucleic Acid Hybridization in the Study of Cell Differentiation;  H. Ursprung Book 1972 Springer-Verlag Berlin Heidelberg 1972 Cell.Nuklein
描述The informational content of cells is encoded in the nucleotide sequences of their DNA. The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription. In the test tube, these restrictions can be exploited for ascertaining similarities and dissimilarities of nucleic acids of varying origin by measuring the kinetics of reassociation of polynucleotides to double-stranded molecules in DNA- DNA renaturation or RNA-DNA hybridization experiments, and by determining the thermal stability and other physical-chemical properties of the resulting hybrid molecules. This method has enormous potential for developmental biology. It offers a more direct approach to the ever-present question of the genetic identity of different cell types in an individual organism, and a more direct test of the hypothesis of diffe- rential gene function. It offers the possibility of localizing genes on chromosomes without the use of Mendelian genetics. It is an indispensable tool in the isolation, purification, and characterization of genes. This volume brings together six articles by investigators actively w
出版日期Book 1972
關(guān)鍵詞Cell; Nukleins?urehybridisierung; Zelldifferenzierung; cell division; developmental biology
版次1
doihttps://doi.org/10.1007/978-3-540-37149-6
isbn_softcover978-3-662-22245-4
isbn_ebook978-3-540-37149-6Series ISSN 0080-1844 Series E-ISSN 1861-0412
issn_series 0080-1844
copyrightSpringer-Verlag Berlin Heidelberg 1972
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Book 1972pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription. In the test tube, these restrictions can be exploited for ascertaining similarities and dissimilarities of nucleic acids of varying origin by measuring the kinetics of reassociation of polyn
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Nucleic Acid Hybridization to Isolated Chromatin,during development. However, because of the possibility of nonspecific binding of added RNA to some material, such as basic proteins (which might also render such spuriously bound RNA resistant to RNase), it has been assumed that purity of the DNA fixed on the nitrocellulose filter is imperative.
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Kinetic Analysis of the Base Sequence Heterogeneity of RNA Molecules by RNA/DNA Hybridization,, after preselection of ribosomal DNA complements from bacteria by RNA/DNA hybridization the renaturation kinetics for these DNA segments indicate that bacterial ribosomal cistrons are similar in base sequence (., 1968).
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Hybridization of Nucleic Acids to Chromosomes,beled rRNA competed effectively against labeled rRNA for binding sites. A number of advances have been made in the last two years. We will report some new results, principally on hybridization to chromosomes of the various classes of .H-RNA, and discuss the prospects of the cytological technique for
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