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Titlebook: Nucleic Acid Aptamers; Selection, Character Günter Mayer Book 2016 Springer Science+Business Media New York 2016 Aptamer generation process

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發(fā)表于 2025-3-21 18:43:58 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Nucleic Acid Aptamers
副標(biāo)題Selection, Character
編輯Günter Mayer
視頻videohttp://file.papertrans.cn/669/668769/668769.mp4
概述Includes cutting-edge methods and protocols for aptamer research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: Nucleic Acid Aptamers; Selection, Character Günter Mayer Book 2016 Springer Science+Business Media New York 2016 Aptamer generation process
描述.This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Practical and easy to follow, .Nucleic Acid Aptamers: Selection, Characterization, and Application. provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems..
出版日期Book 2016
關(guān)鍵詞Aptamer generation process; Aptamer-target interactions; Biophysical properties; Cell surfaces; Nucleic
版次1
doihttps://doi.org/10.1007/978-1-4939-3197-2
isbn_softcover978-1-4939-4934-2
isbn_ebook978-1-4939-3197-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Nucleic Acid Aptamers978-1-4939-3197-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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發(fā)表于 2025-3-22 07:31:43 | 只看該作者
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Applications of Aptamers in Flow and Imaging Cytometryaptamers have gained applications in flow and imaging cytometry for detecting cells expressing distinct antigens. Here we present prospective methods, as a starting point, for using these high-affinity ligands for cytometry applications.
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發(fā)表于 2025-3-22 19:41:21 | 只看該作者
In Vitro and In Vivo Imaging of Fluorescent Aptamersal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).
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發(fā)表于 2025-3-22 23:45:50 | 只看該作者
Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels combined application of the thrombin-specific reversible active-site inhibitor argatroban and the DNA-aptamer HD1-22 for conduction of an enzyme capture assay for reliable measurement of plasma thrombin levels.
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發(fā)表于 2025-3-23 03:11:50 | 只看該作者
Application of Aptamers in Histopathologyand cross-reactivity, aptamers have the potential to revolutionize the field of histopathology due to their high specificity and the ease of their synthesis and modification. Here, we describe a chromogenic staining method for paraffin-embedded tissue sections with FITC-labeled aptamers.
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發(fā)表于 2025-3-23 09:30:51 | 只看該作者
Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/668769.jpg
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