Titlebook: Nucleic Acid Aptamers; Selection, Character Günter Mayer,Marcus M. Menger Book 2023Latest edition The Editor(s) (if applicable) and The Aut

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RNA Capture-SELEX on Streptavidin Magnetic Beadsity. The SELEX protocol has been adapted many times to fit a variety of needs. This protocol describes such an adaptation, namely, RNA-Capture SELEX that we have used to successfully develop small molecule-binding RNA aptamers. Our proposed method specifically selects not only for excellent binding

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The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITEut sequencing after each round of selection. This extension, known as HT-SELEX, results in a vast amount of raw sequencing data that requires tailored bioinformatics approaches to efficiently process and analyze these reads. Here, we present a step-by-step walkthrough of AptaSUITE, a previously publ

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Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetrythe binding thermodynamics and affinity associated with binding. This protocol focuses on ITC applications for studying aptamer interactions with small molecule ligands where ITC has the advantage of being a label-free solution-based technique. The limitation of ITC using a relatively large amount o

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Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScpede K. determination, especially when working with small molecule ligands. These include altered binding behavior caused by ligand immobilization. Further, the compulsory requirement for major differences in size between the bound and unbound state makes small molecule ligands ineligible for separa