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Titlebook: Nuclease Methods and Protocols; Catherine H. Schein Book 2001 Humana Press 2001

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書目名稱Nuclease Methods and Protocols
編輯Catherine H. Schein
視頻videohttp://file.papertrans.cn/669/668747/668747.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Nuclease Methods and Protocols;  Catherine H. Schein Book 2001 Humana Press 2001
描述Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical releva
出版日期Book 2001
版次1
doihttps://doi.org/10.1385/1592592333
isbn_softcover978-1-61737-130-1
isbn_ebook978-1-59259-233-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2001
The information of publication is updating

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Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribstributive, with partially cleaved substrates released to the medium after the initial reaction. Kinetics indicate that either mechanism can be seen in reactions with polymerases, helicases, or nucleases (.). However, many factors affect these kinetics, including the strength of the enzyme-substrate
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Ier1 p: A Kinase and Site-Specific Endoribonucleaselded, covalently modified, and oligomerized with the assistance of specialized ER resident proteins (.). Perturbation of the ER lumen interferes with the production of many essential cellular components and can thus be highly deleterious. Indeed, in humans, defects in protein folding in the ER can l
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Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleaseseaves tRNA. from yeast (.), and Mg., which specifically acts on squid tRNA. (.) to divalent cations complexed to chelating agents, for example Fe.-bleomycin (.) and Cu.-phenanthroline (.) or imidazole (.), to complex nucleic acids and proteins, such as ribozymes (.,.) or the ribonucleoprotein RNaseP
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