Titlebook: Nuclear Reprogramming; Methods and Protocol Nathalie Beaujean,Hélène Jammes,Alice Jouneau Book 2015Latest edition Springer Science+Business

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Studying Bovine Early Embryo Transcriptome by Microarray, genes even if the sample of interest contains small quantities of genetic material. Here we describe the protocols developed by the EmbryoGENE Network to study the bovine transcriptome in early embryo using a microarray experimental design.

Debility

LITHE

不自然

Assessing Reprogramming by Chimera Formation and Tetraploid Complementation,n more, tetraploid complementation. Whether iPS cells in general are functionally equivalent to normal ESCs is difficult to establish. Here, we present the detailed procedure for chimera formation and tetraploid complementation, the most stringent criterion, to assessing pluripotency.

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Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology,thods of probe labeling and hybridization detection are available nowadays. Meanwhile the technique was adapted to be used on many different species and has evolved from a manual to a larger scale and automated procedure. Standardized automated protocols improve the chance to compare different exper

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Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer,tone H2b-GFP expressing mouse embryos, to record cell divisions up to the blastocyst stage. This protocol, which can be adapted to observe other reporters such as Oct4-GFP or Nanog-GFP, allowed us to quantitatively analyze cleavage kinetics of cloned embryos.

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Nuclear Transfer in the Mouse,le manipulation procedure. This protocol describes the isolation and collection of oocytes, treatment of donor cells, visualization of spindle-chromosomal complex, direct injection and enucleation, activation of reconstructed embryos and their in vitro culture and transfer into pseudopregnant mice.

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Nuclear Transfer in Ruminants,t cases the micromanipulation procedures remain the same. However, differences between species require different technical considerations. In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.