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Titlebook: Nuclear Reprogramming; Methods and Protocol Nathalie Beaujean,Hélène Jammes,Alice Jouneau Book 2015Latest edition Springer Science+Business

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書目名稱Nuclear Reprogramming
副標題Methods and Protocol
編輯Nathalie Beaujean,Hélène Jammes,Alice Jouneau
視頻videohttp://file.papertrans.cn/669/668684/668684.mp4
概述Includes fully updated, cutting-edge methods and protocols for nuclear reprogramming.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Nuclear Reprogramming; Methods and Protocol Nathalie Beaujean,Hélène Jammes,Alice Jouneau Book 2015Latest edition Springer Science+Business
描述.Nuclear Reprogramming: Methods and Protocols, Second Edition. includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and up-to-date, .Nuclear Reprogramming: Methods and Protocols, Second Edition. will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.
出版日期Book 2015Latest edition
關鍵詞Epigenetics; Nuclear transfer; Reconstructed embryos; SCNT cloning; Somatic cell genome; Somatic cell nuc
版次2
doihttps://doi.org/10.1007/978-1-4939-1594-1
isbn_softcover978-1-4939-5441-4
isbn_ebook978-1-4939-1594-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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Nuclear Transfer in Rabbit, of the mammalian species considered as difficult to clone. This chapter presents the technical protocol used nowadays to achieve the goal to obtain cloned embryos able to develop to term using fresh somatic cumulus cells.
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