Titlebook: Nuclear Reprogramming; Methods and Protocol Steve Pells Book 2006 Humana Press 2006 Activation.DNA.Ethylen.Monoclonal Antibodies.PCR.Telome

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書目名稱Nuclear Reprogramming影響因子(影響力)




書目名稱Nuclear Reprogramming影響因子(影響力)學(xué)科排名




書目名稱Nuclear Reprogramming網(wǎng)絡(luò)公開度




書目名稱Nuclear Reprogramming網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Nuclear Reprogramming被引頻次




書目名稱Nuclear Reprogramming被引頻次學(xué)科排名




書目名稱Nuclear Reprogramming年度引用




書目名稱Nuclear Reprogramming年度引用學(xué)科排名




書目名稱Nuclear Reprogramming讀者反饋




書目名稱Nuclear Reprogramming讀者反饋學(xué)科排名

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Isolation of Stromal Stem Cells From Human Adipose Tissue,es procedures for isolating millions of highly purified stromal stem cells from human adipose tissue and methods of establishing polyclonal and monoclonal cultures of adipose tissue-derived stem cells.

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Polyethylene Glycol-Mediated Cell Fusion, of cells. Using this method, we have successfully introduced mammalian minichromosomes into a variety of vertebrate cells. The technique described here can be adapted for uses in other cell fusion involved research. This protocol, in principle, provides guidelines for further development of PEG mediated cell fusion technology.

Ossification

Ossification

Pluripotency: ,,soderm and endoderm, respectively. In the future, differentiated cells may be also generated from human ES cells by cultivation of early embryos or from reprogrammed cells derived by nuclear transfer. Such cells could represent potential sources for tissue repair of serious human diseases.

兵團(tuán)

Chromatin Immunoprecipitation Assay for Mammalian Tissues,) allows researchers to determine the status of both histone modifications and the nuclear effector proteins located at gene of interest. ChIP, if applied globally, can also reveal how chromatin structures are dynamically changed when cells respond to certain stimuli. In this chapter, we describe this powerful technique in detail.

helper-T-cells

Nuclear Transplantation in ,,er, the methods used to transplant living nuclei into enucleated eggs of . are described. A method also is described for transplanting multiple somatic cell nuclei into nonenucleated oocytes, a procedure that achieves reprogramming of gene expression in the absence of cell division.

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遺傳學(xué)

Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos,Immunofluorescence using antibodies against 5-methylcytosine is an invaluable method for the large-scale screening of genome-wide methylation. This method has a high degree of reproducibility, essential for the analysis of small numbers of valuable samples, providing information on methylation profiles of individual cells and embryos.