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Titlebook: Nuclear Bodies and Noncoding RNAs; Methods and Protocol Shinichi Nakagawa,Tetsuro Hirose Book 2015 Springer Science+Business Media New York

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發(fā)表于 2025-3-21 18:14:56 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Nuclear Bodies and Noncoding RNAs
副標(biāo)題Methods and Protocol
編輯Shinichi Nakagawa,Tetsuro Hirose
視頻videohttp://file.papertrans.cn/669/668508/668508.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Nuclear Bodies and Noncoding RNAs; Methods and Protocol Shinichi Nakagawa,Tetsuro Hirose Book 2015 Springer Science+Business Media New York
描述.This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful .Methods of Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Nuclear Bodies and Noncoding RNAs: Methods and Protocols .serves as a guideline for further study into the sophisticated regulation of gene expression. .
出版日期Book 2015
關(guān)鍵詞biochemical; cytological; eukaryotes; gene expression; long non-coding RNAs; molecular biological methods
版次1
doihttps://doi.org/10.1007/978-1-4939-2253-6
isbn_softcover978-1-4939-5549-7
isbn_ebook978-1-4939-2253-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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發(fā)表于 2025-3-21 23:01:11 | 只看該作者
Book 2015emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associ
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Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDally visualized by immunostaining for specific marker proteins. Although each type of nuclear body contains a distinct set of proteins, the protein components of most types of nuclear bodies remain to be identified. This chapter introduces a new approach to identify the protein components of specific types of nuclear bodies.
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發(fā)表于 2025-3-22 18:23:34 | 只看該作者
Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of DNA and proteins cross-linked to the RNA of interest. Here, we describe the procedure for CHART and list strategies to optimize nuclear extract preparation, capture oligonucleotide design, and isolation of nucleic acids and proteins enriched through CHART.
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發(fā)表于 2025-3-22 22:52:30 | 只看該作者
Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Maspecific marker proteins. Here we describe in detail the method to identify new nuclear body protein sub-complexes through SILAC (stable isotope labeling by amino acids in culture)-based affinity purification followed by quantitative mass spectrometry.
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發(fā)表于 2025-3-23 04:58:04 | 只看該作者
Super-Resolution Imaging of Nuclear Bodies by STED Microscopyresolution of light microscopy to beyond 100 nm. Here, we describe a super-resolution technique, gated STED, for the analysis of the structure of nuclear bodies, with emphasis on the sample preparation and other technical tips that are important to obtain high-quality super-resolution images.
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發(fā)表于 2025-3-23 09:30:40 | 只看該作者
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