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Titlebook: Nonisotopic Immunoassay; T. T. Ngo Book 1988 Plenum Press, New York 1988 Pet.agriculture.biological.development.environment.enzyme.enzymes

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書目名稱Nonisotopic Immunoassay
編輯T. T. Ngo
視頻videohttp://file.papertrans.cn/668/667311/667311.mp4
圖書封面Titlebook: Nonisotopic Immunoassay;  T. T. Ngo Book 1988 Plenum Press, New York 1988 Pet.agriculture.biological.development.environment.enzyme.enzymes
描述The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA‘s are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician‘s offices, patients‘ homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development
出版日期Book 1988
關(guān)鍵詞Pet; agriculture; biological; development; environment; enzyme; enzymes; fields; infectious disease; iron; mem
版次1
doihttps://doi.org/10.1007/978-1-4684-5466-6
isbn_softcover978-1-4684-5468-0
isbn_ebook978-1-4684-5466-6
copyrightPlenum Press, New York 1988
The information of publication is updating

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Amplification Systems for Enzyme Immunoassayapplied extensively for diagnosing bacterial, viral and parasitic diseases. The enzyme immunoassay method has proved to be a powerful tool in many areas and has been the focus of several reviews, symposia and workshops (Feldmann et al., 1976; Engvall and Pesce, 1978; Malvano, 1980; Guesdon and Avram
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Enzyme Immunoassay Based on Partition Affinity Ligand Assay (PALA) Systemovercome this problem are characterized by elaborate washing procedures. In order to avoid such steps and make immunoassays easier to perform, certain homogeneous assays have been developed (Ullman and Maggio 1980). The present paper deals with yet another approach to immunoassays where the binding
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Immunoenzymatic Assays for Peptide Hormonesd a radiolabeled antibody, each of which recognizes a different antigenic determinant of the analyte (Miles and Hayes, 1968). Since this technique is based on an excess reagent system, it offers several potential advantages over conventional competitive radioimmunoassay methods in terms of speed, se
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Phycobiliproteins as Labels in Immunoassay Hemmila, 1985). As Jolley et al. (1984) have clearly pointed out, the number of photons emitted from a sample of fluorescently tagged molecules can exceed the number of gamma ray photons emitted from the same number of gamma-ray-emitting tagged molecules. The reason for this is two fold: each radio
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