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Titlebook: Nitrogen Fixation; Methods and Protocol Markus W. Ribbe Book 2011 Springer Science+Business Media, LLC 2011 Biogenesis.Biosynthetic mechani

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發(fā)表于 2025-3-21 18:18:53 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Nitrogen Fixation
副標(biāo)題Methods and Protocol
編輯Markus W. Ribbe
視頻videohttp://file.papertrans.cn/667/666668/666668.mp4
概述Compiles a detailed collection of techniques to study one of the most difficult problems in protein biochemistry.Features practical, step-by-step methodologies.Includes key tips and expert implementat
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Nitrogen Fixation; Methods and Protocol Markus W. Ribbe Book 2011 Springer Science+Business Media, LLC 2011 Biogenesis.Biosynthetic mechani
描述.Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.? In .Nitrogen Fixation: Methods and Protocols., recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book.? Written in the highly successful .Methods in Molecular Biology?. series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Practical and cutting-edge, .Nitrogen Fixation: Methods and Protocols. will be useful for anyone interested in nitrogenase research and willing to vent
出版日期Book 2011
關(guān)鍵詞Biogenesis; Biosynthetic mechanisms; Enzymatic mechanisms; Genetics; Metalloenzyme; Nitrogenase; Protein b
版次1
doihttps://doi.org/10.1007/978-1-61779-194-9
isbn_softcover978-1-4939-5841-2
isbn_ebook978-1-61779-194-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

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書目名稱Nitrogen Fixation網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Nitrogen Fixation被引頻次




書目名稱Nitrogen Fixation被引頻次學(xué)科排名




書目名稱Nitrogen Fixation年度引用




書目名稱Nitrogen Fixation年度引用學(xué)科排名




書目名稱Nitrogen Fixation讀者反饋




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沙發(fā)
發(fā)表于 2025-3-21 21:38:54 | 只看該作者
Mechanism of Mo-Dependent Nitrogenaseobal biogeochemical nitrogen cycle, this enzyme has been the focus of intensive research for over 60 years. This chapter provides an overview of the features of nitrogenase as a background to the subsequent chapters of this volume that detail the many methods that have been applied in an attempt to
板凳
發(fā)表于 2025-3-22 03:48:15 | 只看該作者
地板
發(fā)表于 2025-3-22 08:23:21 | 只看該作者
Genomic Analysis of Nitrogen Fixationhave enabled thorough analysis of the gene organization of nitrogen-fixing species, the identification of new genes involved in nitrogen fixation, and the identification of new diazotrophic species. This chapter reviews key characteristics of nitrogen-fixing genomes and methods to identify and analy
5#
發(fā)表于 2025-3-22 12:34:26 | 只看該作者
Enzymatic Systems with Homology to Nitrogenaseorophyllide during chlorophyll biosynthesis. Formation of bacteriochlorophyll additionally requires a structurally related enzyme system which is termed chlorophyllide oxidoreductase (COR). During DPOR catalysis, the homodimeric subunit ChlL. transfers electrons to the corresponding heterotetrameric
6#
發(fā)表于 2025-3-22 13:28:42 | 只看該作者
Molecular Biology and Genetic Engineering in Nitrogen Fixationdied diazotrophs, . is the best studied for its convenience of aerobic growth, its high levels of nitrogenase expression, and its genetic tractability. This chapter includes protocols and strategies in the molecular biology and genetic engineering of . that have been used as valuable tools for advan
7#
發(fā)表于 2025-3-22 18:33:43 | 只看該作者
Purification of Nitrogenase Proteinsree metal centers that are critical for nitrogenase activity. The inherent complexity of this enzyme system, which is further compounded by the sensitivity of the metal clusters toward oxygen, makes the large-scale purification of fully active nitrogenase proteins a formidable task. This chapter hig
8#
發(fā)表于 2025-3-22 22:31:43 | 只看該作者
Assays of Nitrogenase Reaction Productse (MgATP), a suitable source of reductant, a buffer system compatible with the product-quantification protocol to be used, and the desired substrate. The assay is initiated by injection of the component protein(s) of the enzyme or MgATP and terminated by injection of either acid or a solution of Na.
9#
發(fā)表于 2025-3-23 01:25:06 | 只看該作者
Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductasegymnosperms, ferns, algae, and photosynthetic bacteria. During protochlorophyllide (Pchlide) reduction, the homodimeric subunit ChlL. of DPOR transfers electrons on the corresponding heterotetrameric catalytic subunit (ChlN/ChlB).. Although DPOR shares significant amino acid sequence homology to the
10#
發(fā)表于 2025-3-23 09:36:51 | 只看該作者
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