Titlebook: Nitric Oxide Protocols; Aviv Hassid Book 2004Latest edition Humana Press 2004

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Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Specte simultaneously stable-isotope-labeled nitrite and nitrate (i.e. [.N]nitrite and [.N]nitrate), which are exclusively produced by NOS-catalyzed oxidation of .-[.-.N.]-arginine, the stable-isotope-labeled .-arginine analog. .NOS may concomitantly produce .NO and O.., thus forming peroxynitrite (ONOO.

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Measurement of Nitric Oxide-Related Enzymes in the Brain by , Hybridization,se (AL) mRNA expression in the rat brain to illustrate the methods allowing the nonradioactive localization of transcripts by ISH, coupled to immunohistochemistry to identify the specific cell types of central nervous system (CNS) on the same section.

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Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conss measured by in vivo microdialysis. Blood pressure and heart rate were monitored by a radiotelemetry system in a conscious state. In the AdeNOS-infected rats, blood pressure and heart rate significantly decreased from d 5 to d 10, and then gradually recovered over time. These methods should be usef

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Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer,include relatively high transduction efficiencies, both replicating and nonreplicating cells may be infected, and the high titers of adenovirus that can be produced. The methods described in this chapter include the cloning of the iNOS cDNA into a recombinant adenoviral vector, large-scale productio

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thoroughly described elsewhere, i.e., [1-5], and a general treatment will not be included here. A few other authors have presented work regarding design automation for SI circuits: Hughes et. al. developed switched-current cells for design automation [6] and an automated design system for SI filters