Titlebook: Next Generation Culture Platforms for Reliable In Vitro Models; Methods and Protocol Tiziana A.L. Brevini,Alireza Fazeli,Kursad Turksen Boo

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Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissuesropriate biological signals and mechanical properties of the original tissue. Here, we describe different methods to create viable scaffolds from decellularized heart and liver as useful tools to study and exploit ECM biological key factors for the generation of engineered tissues with enhanced regenerative properties.

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3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids,eated from HepG2/C3A human hepatocytes or coculture of HepG2/C3A and endothelial cell line HMEC-1. It includes protocol for spheroids disassembling, labeling of cells with fluorescein diacetate and propidium iodide and instructions for flow cytometry gating optimized for analysis of heterogeneous cell populations form spheroids.

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Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometrcomet assay, transcriptomics, proteomics and lipidomic. This fast and gentle disassembly of intact spheroids into rim and core fractions, and further into viable single-cell suspension provides an opportunity to bridge the gap from 3D cell culture to current state-of-the-art analysis methods.

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