Titlebook: New Technologies for Glutamate Interaction; Neurons and Glia Maria Kukley Book 2024 The Editor(s) (if applicable) and The Author(s), under

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Coram Guevara,Rodrigo Varas,María Cecilia Angulo,Fernando C. Ortiz

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Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encodedon, mechanism of action, in vivo imaging, data analysis, and future directions. We include detailed protocols for iGluSnFR imaging in common preparations (bacteria, cell culture, and brain slices) and model organisms (worm, fly, fish, rodent).

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Ligand-Directed Chemical Labeling for Visualizing and Analyzing AMPA Receptors in Neuronsned with bioorthogonal click chemistry. This modified method is suitable for quantifying the localization and trafficking of AMPARs. This chapter provides the experimental and technical details for visualizing and analyzing AMPARs using these chemical-labeling methods in cultured neurons.

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Live FRET-FLIM Imaging to Study Metabotropic Signaling via the NMDA Receptorl NMDAR binding compounds on the NMDAR intracellular conformation or to study signaling proteins implicated in ion-flux-independent synaptic depression. Moreover, one could adapt these procedures to study any kind of protein-protein interaction and its dynamics in living cells.

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Quantitative Analysis of Single Glutamatergic Vesicles in the Brainseconds to seconds) and thus are too slow for detection of single sub-millisecond exocytosis events, we here present the technological development of a novel ultrafast (20?kHz) enzyme-based gold nanoparticle-coated amperometric microsensor designed for detection of glutamate release from synaptic ve

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Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapsesces, it has been successfully demonstrated that astrocytic Gq- and Gi-DREADD modulation induces long-term synaptic potentiation. Finally, we will elaborate on other approaches to study chemogenetic modulation of astrocytes at glutamatergic synapses.

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Analysis of Synaptic Glutamate Clearance as a Possible Indicator of Synaptic Health in the Degenerat expression of a modified version of the glutamate transporter EAAT2, the type 2 excitatory amino acid transporter. Our focus was on improvement of glutamate clearance, since it has repeatedly been suggested that there might be an excitotoxic component in the pathogenesis of HD. From the very outset

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Mapping Synaptic Inputs to Oligodendroglial Cells Using In Vivo Monosynaptic Viral Tracing information in different contexts and the function of these connections. Here, we summarize a useful synaptic tracing method, monosynaptic retrograde rabies tracing, and how it can be adapted for mapping synaptic inputs to oligodendroglial cells.