Titlebook: New Protein Techniques; John M. Walker Book 1988 Springer Science+Business Media New York 1988

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CAMP

Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique,resolution of complex protein mixtures. Although Coomassie Brilliant Blue R250 has proved ideal as a general protein stain for the more traditional applications of this method, current trends toward thinner gels, decreased sample loading (to improve resolution), and the recent developments of two-di

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Chromatofocusing,aboratory work as well as in biochemical research. One crucial part of such investigations is the application of a suitable method in order to separate the individual proteins and/or resolve a certain protein into subtypes or microheterogeneous components. There are two major categories of procedure

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Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels,amide gels containing immobilized pH gradients (IPGs). The Immobilines are a series of seven acrylamide derivatives with the general structure: .where R contains either a carboxyl (acidic Immobilines) or a tertiary amino (basic Immobilines) group, forming a series of buffers with different p. values

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Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension, of choice for the analysis of complex protein mixtures (.–.). In the standard procedure, cylindrical tube isoelectric focusing (IEF) gels are used. Unfortunately this system suffers from severe cathodic drift resulting in pH gradients that do not extend above pH 8, resulting in concomitant loss of

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Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First their high resolution capacity. This resolving power is maximized in high-resolution, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) based on the technique developed by O’Farrell (.). This methodology involves a combination of a first-dimension separation in cylindrical isoelectric f