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Titlebook: Neutrophil Methods and Protocols; Mark T. Quinn,Frank R. DeLeo Book 2014Latest edition Springer Science+Business Media, LLC 2014 NADPH oxi

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發(fā)表于 2025-3-21 17:32:25 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Neutrophil Methods and Protocols
編輯Mark T. Quinn,Frank R. DeLeo
視頻videohttp://file.papertrans.cn/665/664702/664702.mp4
概述Provides neutrophil protocols that are broadly applicable to leukocyte biology.Includes protocols for isolation from humans and animal species.Presents an interesting and informative overview of the f
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Neutrophil Methods and Protocols;  Mark T. Quinn,Frank R. DeLeo Book 2014Latest edition Springer Science+Business Media, LLC 2014 NADPH oxi
描述.Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. .Neutrophil Methods and Protocols., .Second Edition. provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of .in vivo. transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the
出版日期Book 2014Latest edition
關鍵詞NADPH oxidase; bactericidal activity; gene expression; human neutrophil; neutrophil; neutrophil apoptosis
版次2
doihttps://doi.org/10.1007/978-1-62703-845-4
isbn_softcover978-1-4939-6062-0
isbn_ebook978-1-62703-845-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2014
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 22:41:13 | 只看該作者
Neutrophil Isolation from Nonhuman Speciesanimals (bovine, equine, ovine), small animals (murine and rabbit), and nonhuman primates (cynomolgus macaques), and describe optimized details for obtaining the highest cell purity, yield, and viability. We also describe methods to verify phagocytic capacity in the purified cell populations using a flow cytometry-based phagocytosis assay.
板凳
發(fā)表于 2025-3-22 03:28:32 | 只看該作者
Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafishbe technically challenging. In this chapter, we describe the practical aspects of imaging neutrophils in zebrafish embryos using spinning disk confocal microscopy. Similar setups can also be applied to image other motile cell types and signaling processes in translucent animals or tissues.
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發(fā)表于 2025-3-22 07:01:43 | 只看該作者
Detection of Bidirectional Signaling During Integrin Activation and Neutrophil Adhesionion that facilitate the transition from neutrophil rolling to arrest. Methodology is presented to correlate the relationship between integrin conformation, bond formation with ICAM-1, and cytoskeletal engagement and adhesion strengthening necessary to achieve a migratory phenotype.
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Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca. in neutrophils.
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發(fā)表于 2025-3-22 18:45:18 | 只看該作者
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發(fā)表于 2025-3-23 00:31:57 | 只看該作者
Book 2014Latest editionecond Edition. provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of
9#
發(fā)表于 2025-3-23 02:04:50 | 只看該作者
1064-3745 es.Presents an interesting and informative overview of the f.Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. .Neutrophil Methods and Protocols., .Second Edition. provides a concise set of protocols written by leading researchers in the f
10#
發(fā)表于 2025-3-23 05:53:37 | 只看該作者
Mark T. Quinn,Frank R. DeLeoProvides neutrophil protocols that are broadly applicable to leukocyte biology.Includes protocols for isolation from humans and animal species.Presents an interesting and informative overview of the f
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