Titlebook: Neurotransmitter Methods; Richard C. Rayne Book 1997 Humana Press 1997

CYN

Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method,ents are performed using brain slices soon after their preparation. Thin brain slices have limited viability: Unless special culture conditions are employed, slices die after 1 d in vitro (DIV) (..). Longer viability is often desired to permit study of more extended processes, such as long-term modu

Encapsulate

命令變成大炮

Synaptosomes,sealed particles that contain small, clear vesicles and sometimes larger densecore vesicles, indicating their presynaptic origin. Occasionally, fractions of electron-dense, postsynaptic membranes remain attached to the synaptosomes, facing the active zone. Evidently, synaptosomes contain all the com

ELUDE

Synaptoneurosomes,e not suited for the investigation of synaptic events mediated by postsynaptic mechanisms. In the past, isolated membrane fractions enriched in postsynaptic densities have been used for this purpose (.). However, this fraction of postsynaptic densities is not appropriate to study receptor-mediated s

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Preparation of Synaptosomal Plasma Membranes by Subcellular Fractionation,res some purification in order to study neuronal and glial functioning in isolation at the molecular level. The introduction of the ultracentrifuge by Svedberg in 1925 offered neurochemists for the first time the ability to fractionate homogenized brain tissue into its subcellular components. Furthe

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Detection of Neuropeptides by Immunocytochemistry,t neuropeptides have important roles as neurohormones, neuromodulators, cytokines, morphogenetic factors, and possibly in some cases, as true neurotransmitters. Each neuropeptide may even be multifunctional and exist in several isoforms in a given animal species. In the search for functions of neuro

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Anecdote

cGMP-Immunocytochemistry,protein antigens are relatively insensitive to the type of fixative used. Apparently, the epitopes are not altered in a decisive way by the fixative, immobilization is usually effective, and more than one eptiope may be recognized by polyclonal antisera. In studying by ICC the location of small, hyd

CHARM

Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue,tuations, at varying concentrations and with virtually all tissues, it has remained one of the most widely used fixatives. Formaldehyde is a reactive electrophilic species (.,.) that reacts readily with primary amines, amide groups, aromatic rings, and alcoholic hydroxyl groups, crosslinking various

sperse

Localization of Nitric Oxide Synthase Using NADPH Diaphorase Histochemistry,990, the enzyme responsible for synthesis of NO in the brain, NO synthase (NOS), was purified and antibodies to it were used to establish the distribution of NO-producing neurons in the rat brain (.). NOS was also found to be responsible for an enzyme activity in the brain known as NADPH diaphorase