Titlebook: Neuronal Morphogenesis; Methods and Protocol Kazuhito Toyooka Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive li

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N斯巴達(dá)人

Quantifying Dendritic Arbors In Vitro and In Vivo in Rodent Models,endritic arbor in primary cell cultures and in the intact rodent brain. These techniques can be used to answer significant scientific questions, such as the effects of disease processes, drugs, growth factors, and diverse environmental stressors on dendritogenesis in both in vitro and in vivo rodent models.

細(xì)菌等

Analyses of Neurite and Spine Formation by In Utero Electroporation in Mice,veloping mouse embryos in the uterus. By combining with the Cre–loxP system, the morphology of individual neurons can be clearly and sparsely visualized. Here, we describe how this labeling system can be applied to visualize and evaluate the dendrites and dendritic spines of cortical neurons.

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Mast-Cell

DNeuroMAT: A Deep-Learning-Based Neuron Morphology Analysis Toolbox, when handling the huge volume of whole brain microscopy imaging data. Here, we present a .eep-learning-based .n .orphology .nalysis .oolbox (DNeuroMAT) for automated analysis of neuron microscopy images, which consists of three modules: neuron segmentation, neuron reconstruction, and neuron critical points detection.

輕信

Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Uteroentify neurite morphology. To understand the dynamics of neurite structure at early stages of neurite formation, we describe in this chapter ex vivo live imaging using a confocal microscope at P0 in combination with in utero electroporation (IUE).

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漂白

Electron Microscopy of Neurons on Biomimetic Substrates,ectron microscopy approaches, owing to their ability of discerning the details of neural morphogenesis at a nanoscale resolution, may play a crucial role in unravelling the fine ultrastructure of neurons interfacing with biomimetic structured substrates.

欲望

Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy,itatory synapses in the central nervous system, and underlying molecular interactions/biochemical reactions using two-photon fluorescence lifetime microscopy (2P-FLIM) in combination with F?rster resonance energy transfer (FRET)-based biosensors.

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