Titlebook: Neuronal Cell Death; Methods and Protocol Arezu Jahani-Asl Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen

燦爛

Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Cortical Stroke with Persistenal ischemic cortical stroke by injecting L-NAME, another potent vasoconstrictor, in combination with ET-1 into the sensorimotor cortex. This ET-1/L-NAME stroke induction protocol produces consistent focal cortical infarcts and sensorimotor functional impairments in C57BL/6 mice.

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Modeling Axonal Degeneration Using Motor Nerve Organoids,n?axon degeneration can be rapidly induced with chemical damages. Neuroprotective effects of compounds can be examined using the degenerated axons. This motor neuron axon bundle degeneration model should facilitate future screening for drugs against diseases affecting axon fascicles.

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Spinal Cord Injury in the Mouse Using the Infinite Horizon Spinal Cord Impactor,d of loss of bladder function, weight loss, and paralysis of the hind limbs, with more severe injuries resulting in further deficits. This chapter describes the surgical protocol along with pre- and postoperative care, as well as mitigation strategies for any setbacks that might occur.

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動(dòng)脈

Assessment of Dopaminergic Neurodegeneration in Mice,ntia nigra pars compacta, a brain area prone to neuron loss in PD. In addition, we outline the protocol for the analysis of the dopaminergic terminals in the striatum, the projection area of midbrain dopaminergic neurons, as a readout for the integrity of the nigrostriatal projections.

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Isolation of Mouse Embryonic Neural Stem Cells and Characterization of Neural Stem Markers by Flow loped which serve as invaluable tools to investigate the early mechanisms of disease pathogenesis. In this chapter, we describe our optimized method to obtain and maintain reproducible neurosphere cultures from transgenic or patient mouse models followed by characterization of NSCs by flow cytometry.

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intellect

Arezu Jahani-AslIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts