Titlebook: Neuronal Cell Culture; Methods and Protocol Shohreh Amini,Martyn K. White Book 2021Latest edition Springer Science+Business Media, LLC, par

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Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates,ation and targeting, we have sought to promote neurite outgrowth by refining the techniques of growing DRG neurons in culture. This chapter describes detailed methods for the dissection and purification of DRG neurons and quantitative assessment of neurite on promoting or inhibitory substrates.

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Transfection of Neuronal Cultures,, still remains a challenging task. Here, we overview the advantages and disadvantages of various techniques for the transfection of primary neurons, and provide an optimized protocol for FuGENE-6 (Promega) which allows for a suitable transfection efficiency of primary neuronal cultures.

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General Overview of Neuronal Cell Culture, field who are currently using state-of-the-art techniques to advance our understanding of the molecular and cellular biology of the central nervous system. Each chapter provides detailed descriptions and experimental protocols for a variety of techniques ranging in scope from basic neuronal cell line culturing to advanced and specialized methods.

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Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12, and SK-N-MC,onal NT2 cells, and the differentiation, transduction, and transfection of human SK-N-MC cells and rat PC12 cells to obtain cells with the morphology of differentiated neurons that can express exogenous genes of interest at high level.

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Murine Teratocarcinoma-Derived Neuronal Cultures,into serotonergic or catecholaminergic neurons. The protocols described herein can be used for dissection of the pathways such as gliogenesis and neurogenesis that are involved in differentiation of pluripotent stem cells such as F9 and P19 into glial cells or terminally differentiated neurons.

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