Titlebook: Neurodegeneration Methods and Protocols; Jean Harry,Hugh A. Tilson Book 1999 Humana Press 1999

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Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique,les (.). Normal physiological functions of the cell are regulated by changes in intracellular free Ca. ([Ca.].), which ranges from 0.1 to 0.3 μ.. This low concentration is regulated by energy-dependent transport systems located in plasma membrane, endoplasmic reticulum, and mitochondria (.). Cytosol

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Measurement of Calcium Buffering by Intracellular Organelles in Brain, intracellular organelles, such as mitochondria and endoplasmic reticulum (microsomes) (.,.). Under normal conditions, Ca. enters the cell by diffusion down an electrochemical gradient through voltage-dependent or receptor-mediated Ca.-sensitive channels (.). Calcium can also be released from intra-

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Receptor-Mediated Release of Inositolphosphates in Brain Slices,ivation of phospholipase C (PLC) and the rapid hydrolysis of phosphoinositides (.–.). The activation of PLC by receptors for most neurotransmitters and growth factors occurs through a mechanism involving a guanine nucleotide regulatory protein or G protein. The PLC-catalyzed hydrolyses of phosphatid

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The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions, commonly used, including precipitation assays using antibody/antigen complexes, agglutination assays using coated erythrocytes or other particles, and radio-(RIA) and enzyme immunoassays. Multiple factors determine the best assay for a particular use, including sample concentration, technical diffi

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Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders,.). The mechanisms triggering cell death and synaptic damage in these disorders might be related to gain of a toxic property and/or loss of neuroprotective capacity of a specific neuronal protein (.). Many of these neuronal molecules play an important role in the maintenance and functioning of the s

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1543-1894 led to an increasing interest in utilizing these approaches in neurobiological research. Efforts in the most rapidly advancing areas are multidisciplinary and the o- come of contributions from many investigators employing a variety of te- niques to address a specific problem. Although a strong basi

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Subtractive Hybridization of cDNA Libraries,ultures or even tissues have been analyzed by subtractive hybridization with success. The magnitude of success is directly related to the magnitude of the differential upregulation of relevant transcripts in the activated state over the unactivated state.

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