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Titlebook: Neural Progenitor Cells; Methods and Protocol Loic P. Deleyrolle Book 2022Latest edition Springer Science+Business Media, LLC, part of Spri

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發(fā)表于 2025-3-21 20:05:02 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Neural Progenitor Cells
副標(biāo)題Methods and Protocol
編輯Loic P. Deleyrolle
視頻videohttp://file.papertrans.cn/664/663734/663734.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Neural Progenitor Cells; Methods and Protocol Loic P. Deleyrolle Book 2022Latest edition Springer Science+Business Media, LLC, part of Spri
描述This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Neural Progenitor Cells: Methods and Protocols, Second Edition. serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology..
出版日期Book 2022Latest edition
關(guān)鍵詞Neural stem cells; Stem cell-based therapeutics; Central nervous system ailments; Brain microenvironmen
版次2
doihttps://doi.org/10.1007/978-1-0716-1783-0
isbn_softcover978-1-0716-1785-4
isbn_ebook978-1-0716-1783-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
The information of publication is updating

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發(fā)表于 2025-3-21 21:05:56 | 只看該作者
Microglial FACS for Robust RNA Recovery for Next-Generation Sequencing,ells has its challenges. Here we describe a method to enrich CD11b?+?microglial cells from brain tissue followed by FACS, for a reliable and reproducible method for the recovery of high-quality RNA from sorted microglia for downstream sequencing.
板凳
發(fā)表于 2025-3-22 03:35:10 | 只看該作者
Isolating and Culturing of Precursor Cells from the Adult Human Brain,s a guideline for dissociation of adult human brain tissue from biopsy or autopsy specimens. While protocols for subsequent culturing of neural precursors are included, the main focus is the preparation of a suspension of viable single cells that may also be useful in other experimental paradigms, such as genomic profiling.
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發(fā)表于 2025-3-22 05:42:54 | 只看該作者
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發(fā)表于 2025-3-22 09:30:44 | 只看該作者
Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region,NSA) as a methodology that allows to efficiently isolate, expand, and differentiate somatic NSCs derived from the postnatal and adult forebrain periventricular region while preserving proliferation, self-renewal, and multipotency, the main attributes that provide their functional identification.
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Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord,tients who had agreed before passing to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.
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發(fā)表于 2025-3-23 02:37:07 | 只看該作者
Generating Cerebral Organoids from Human Pluripotent Stem Cells,ere we describe a protocol for the differentiation of hPSCs to human cerebral organoids using a commercially available kit (STEMdiff? Cerebral Organoid Kit) and discuss methods to scale up the protocol in a high-throughput manner.
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發(fā)表于 2025-3-23 08:29:33 | 只看該作者
Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseneurologists, neuropathologists, and research coordinators to guarantee that donated tissue is properly processed and archived. If properly run, brain banks can ultimately lay the foundation for new brain research and pioneer the discovery of new therapies for a variety of neurological diseases.
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