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Titlebook: Nanoscale Imaging of Synapses; New Concepts and Opp U. Valentin N?gerl,Antoine Triller Book 2014 Springer Science+Business Media New York 2

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發(fā)表于 2025-3-21 19:33:08 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Nanoscale Imaging of Synapses
副標(biāo)題New Concepts and Opp
編輯U. Valentin N?gerl,Antoine Triller
視頻videohttp://file.papertrans.cn/661/660935/660935.mp4
概述Timeliness – Superresolution methods are beginning to transform neurobiological research.Relevance – Progress in synapse research hinges on advances in superresolution imaging.Competence – Chapters ar
叢書名稱Neuromethods
圖書封面Titlebook: Nanoscale Imaging of Synapses; New Concepts and Opp U. Valentin N?gerl,Antoine Triller Book 2014 Springer Science+Business Media New York 2
描述Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.
出版日期Book 2014
關(guān)鍵詞live-cell fluorescence microsopy; nanoscopy; neurons and glia; physiology; synapses
版次1
doihttps://doi.org/10.1007/978-1-4614-9179-8
isbn_softcover978-1-4939-4918-2
isbn_ebook978-1-4614-9179-8Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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發(fā)表于 2025-3-21 20:39:21 | 只看該作者
Flash-and-Freeze Electron Microscopy: Coupling Optogenetics with High-Pressure Freezing,s. We demonstrated that synaptic vesicle fusion intermediates could be captured using this technique. This method can be readily applied to other light-activatable molecules, such as caged compounds, light-switchable ligands, and photoactivatable proteins, to study dynamic changes in cells.
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Electron Tomography for the Study of Synaptic Ultrastructure in Fixed Brain Sections,e describe an approach to the study of ultrastructure that relies on plastic-embedded aldehyde-fixed material stabilized with tannic acid instead of osmium tetroxide. This approach offers a new window into the structural basis of synaptic processing in the mammalian brain.
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Book 2014ion in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.
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發(fā)表于 2025-3-22 13:21:13 | 只看該作者
Book 2014ease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even dow
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Nanoscale Imaging of Synapses978-1-4614-9179-8Series ISSN 0893-2336 Series E-ISSN 1940-6045
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