Titlebook: NLR Proteins; Methods and Protocol Pablo Pelegrín,Francesco Di Virgilio Book 2023Latest edition The Editor(s) (if applicable) and The Autho

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Method to Measure Ubiquitination of NLRs,tin system has recently emerged as a new level of regulation of the inflammasome complex. Here we describe a method to detect poly-ubiquitination of NRLP3 using two different approaches: (i) detection with a ubiquitin antibody or (ii) using TUBEs (Tandem Ubiquitin Binding entities). This approach ca

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Methods to Study NLR in Human Blood Cells, cases, the clinical manifestations are due to increased activity of the NLRP3 inflammasome resulting in increased IL-1β secretion. Investigating inflammatory cells from subjects affected by autoinflammatory diseases presents a number of technical difficulties related to the rarity of the diseases,

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