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Titlebook: Microinjection; Methods and Applicat David J. Carroll Book 2009 Humana Press 2009 Antibodies.Expression.Gene function.Nucleotide.Protein ex

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發(fā)表于 2025-3-21 18:40:12 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Microinjection
副標(biāo)題Methods and Applicat
編輯David J. Carroll
視頻videohttp://file.papertrans.cn/634/633292/633292.mp4
概述Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells.Contains detailed m
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Microinjection; Methods and Applicat David J. Carroll Book 2009 Humana Press 2009 Antibodies.Expression.Gene function.Nucleotide.Protein ex
描述.As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In .Microinjection: Methods and Applications., expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in .Xenopus. oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful .Methods in Molecular Biology.? series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls
出版日期Book 2009
關(guān)鍵詞Antibodies; Expression; Gene function; Nucleotide; Protein expression; RNA expression; Regulation; gene exp
版次1
doihttps://doi.org/10.1007/978-1-59745-202-1
isbn_softcover978-1-4939-6090-3
isbn_ebook978-1-59745-202-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

書目名稱Microinjection影響因子(影響力)




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書目名稱Microinjection網(wǎng)絡(luò)公開度學(xué)科排名




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地板
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Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans,intracytoplasmic sperm injection (ICSI) in humans. The following protocols will provide a detailed description of how to perform immunodetection and imaging of human eggs, zygotes, and sperm by fluorescence (confocal and epifluorescence) and electron microscopy.
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,Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs, The use of luciferase to monitor protein expression after injection of cRNA is a sensitive and effective method that efficiently allows for sets of eggs to be used for PLCζ quantitation, Ca. imaging, and studies of embryo development.
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發(fā)表于 2025-3-22 15:42:24 | 只看該作者
Analysis of 14-3-3 Family Member Function in , Embryos by Microinjection of Antisense Morpholino Olng 14-3-3 τ exhibited gastrulation and axial patterning defects, but embryos lacking 14-3-3 γ exhibited eye defects without other abnormalities, and embryos lacking 14-3-3 ζ appeared completely normal. These and other results demonstrate the power and specificity of the morpholino antisense oligo microinjection technique.
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發(fā)表于 2025-3-22 19:41:17 | 只看該作者
Quantitative Microinjection of Mouse Oocytes and Eggs, an inverted microscope. The techniques are applicable to the oocytes, eggs, and early embryos of most mammalian species. Included are some general instructions on fabrication of transfer pipettes, holding pipettes, beveled injection pipettes, and equipment for quantitative injection.
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發(fā)表于 2025-3-22 23:55:22 | 只看該作者
1064-3745 bditis elegans, and tissue culture cells.Contains detailed m.As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In .Microinjection: Methods and Applica
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A Microinjectable Biological System, the , Oocyte, as an Approach to Understanding Signal Transduct of the cell cycle and easily amenable to biochemical, electrophysiological, and cytological studies. We describe how to microinject proteins or peptides in this model and we study, as an example, the Grb2 transduction cascade.
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