Titlebook: Microfluidics Diagnostics; Methods and Protocol Valérie Taly,Stéphanie Descroix,Karla Perez-Torall Book 2024 The Editor(s) (if applicable)

fluoroscopy

Book 2024ficant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell

Hallmark

Toxoid-Vaccines

Anemia

A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level in detail, building on the published droplet-based microfluidic platform DropMap. We give an updated, detailed guide toward quantifying protein secretion rates, discussing its setup and limitations. We illustrate the protocol on two key immunological analytes, immunoglobulin G, and interferon-γ.

消耗

Microfluidic 3D Cytotoxic Assayectively demonstrates the advantages of 3D microfluidic platforms in closely recapitulating immune responses within the tumor microenvironment. Moreover, a wide range of experimental possibilities and applications using microfluidic 3D cytotoxic assays is introduced in this chapter, along with their capabilities, limitations, and future outlook.

大范圍流行

Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and N limited access to resources and infrastructure prevails or rapid clinical decision-making is critical. These microfluidic systems exploit physical and chemical phenomena, such as capillary forces and surface tension, to manipulate tiny volumes of fluids without the need for external power sources,

不透氣

DNA Sizing with pg/μL Sensitivity for Cell-Free DNA Analysis with the μLAS Technology cell-free DNA (cfDNA) extracted from blood plasma. Operated on commercial systems for capillary electrophoresis, we provide the size distribution of healthy individuals or patients using an input of 10?μL.

Pandemic

On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samplesng and personalized medicine. However, the isolation of cfDNA from peripheral blood has remained a challenge due to the low abundance and high fragmentation of these molecules. Here, we present a dynamic Magnetic ExTRactiOn (METRO) protocol using microfluidic fluidized bed technology to isolate circ

兵團

Organization

Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)atments. However, due to their rarity among the multitude of blood cells, it remains a challenge to recover them alive with high level of purity, i.e., with few remaining white blood cells, and in a time frame compatible with the clinical context. Microfluidic chips have emerged as promising tools t