Titlebook: Microbial Steroids; Methods and Protocol Carlos Barreiro,José-Luis Barredo Book 2023Latest edition The Editor(s) (if applicable) and The Au

Spinous-Process

978-1-0716-3387-8The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

小教堂

BLOT

Book 2023Latest editionnd perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, a

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Selection of Biodegrading Phytosterol Strainshe products in the highest proportion identified for each trial are androstenedione and androstadienedione. The results suggest that the high substrate concentration could be a selective mechanism to obtain strains more efficient in the biotransformation of β-sitosterol into steroidal bases.

連鎖,連串

規(guī)章

RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics the analysis can be provided by commercial or central services. Here, we detail a protocol for the first part of the analysis, the RNA extraction and an introductory protocol to the bioinformatics analysis of the sequencing data to generate the differential expression results.

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SYN

Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Choll bacteria able to degrade sterols, bile acids, and androgens is shown. Moreover, procedures for the determination of cholesterol oxidase or different hydroxysteroid dehydrogenase activities in . DOC21, . sp. HE24.12, . sp. HE24.4J and . sp. HE24.3 are also detailed.

accomplishment

Bidimensional Analyses of the Intra- and Extracellular Proteomes of Steroid Producer Mycobacterianversion, are the core of the present chapter. Both, monodimensional gels, as preparatory analysis, and bidimensional gels as proteome analysis are described. As a proof of concept, the protein extraction methods for both sub-proteomes of . are described. Thus, procedures and relevant key points of these proteome analyses are fully detailed.

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Fungal Sterol Analyses by Gas Chromatography–Mass Spectrometry Using Different Derivativesorm and the culture conditions (liquid and solid media). The different methodologies that can be used to obtain free sterols, acetate, and/or TMSi derivatives are presented. Identification keys using the fragmentation at 70?eV are also described.