Titlebook: Microbial Proteomics; Methods and Protocol D?rte Becher Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Subcel

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書目名稱Microbial Proteomics影響因子(影響力)




書目名稱Microbial Proteomics影響因子(影響力)學(xué)科排名




書目名稱Microbial Proteomics網(wǎng)絡(luò)公開度




書目名稱Microbial Proteomics網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Microbial Proteomics被引頻次




書目名稱Microbial Proteomics被引頻次學(xué)科排名




書目名稱Microbial Proteomics年度引用




書目名稱Microbial Proteomics年度引用學(xué)科排名




書目名稱Microbial Proteomics讀者反饋




書目名稱Microbial Proteomics讀者反饋學(xué)科排名

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Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)les. Once set up, the workflow relies on defined chemical or commercially available complex media leading to universal heavy labeling by the stable isotope .N. This chapter provides a detailed protocol for labeling of different bacteria and the strategy for evaluating the incorporation rate of the heavy stable isotopes in the sample is discussed.

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In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Sengs and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4?×?10. internalized bacterial cells as starting material.

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意外的成功

https://doi.org/10.1007/978-1-4939-8695-8Subcellular fractionation; Protein quantification; Metaproteomics; Microbes; Proteome analysis; Post-tran

Anticoagulants

D?rte BecherIncludes cutting-edge methods and protocols for the study of microbial proteomics.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the

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Next-Generation Trapping of Protease Substrates by Label-Free Proteomicsd, concentrated by an 1D-short gel, and identified by LC-coupled mass spectrometry (LC-MS) followed by label-free quantification. The identification of four known FtsH substrates validated this approach and suggests that it is generally applicable to AAA. proteases.