Titlebook: Microbial Metabolomics; Methods and Protocol Edward E.K. Baidoo Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

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Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysisof which describing new biomarkers better elucidating microbial disease mechanisms and improved diagnostics and treatment thereof. Here, we describe a metabolomics method for extracting the total metabolome (all compounds present in the microbial cell irrespective of the compound class), for analysi

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Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Interaction Liquid Chromatographyh precision and accuracy. This chapter presents a robust HILIC-ESI-MS/MS procedure for targeted quantitative profiling of more than 50 polar key metabolites in multicomponent endogenous extracts. Without using ion-pairing-agents or prior derivatization protocols, organic acids, amino acids, sugar ph

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Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in using two primary building blocks, namely, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) that are generated via the mevalonate (MVA) or deoxy-.-xylulose-5-phosphate (DXP) pathways. Isoprenoid biosynthetic pathways are prevalent in eukaryotes, archaea, and bacteria. Measurement

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Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profitate lead compound development and to anticipate potential resistance mechanisms that might emerge. Here we describe a mass spectrometry-based approach for detecting metabolic changes in asexual erythrocytic stages of . induced by antimalarial compounds. Time-resolved or concentration-resolved meas

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