Titlebook: MicroRNA Protocols; Shao-Yao Ying Book 2013Latest edition Springer Science+Business Media, LLC 2013 Drug agent development.Expression prof

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MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells,ls. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles during the course of P19 cells neuronal differentiation. The results indicated that there were significant differences between the miRNA expression in P19 EC cells and the resultant differentiated neural stem c

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Expression Pattern Analysis of MicroRNAs in ,,nscriptional level by basepairing with target messenger RNA and blocking its translation or inducing its degradation. miRNAs play important roles in a variety of biological processes, including development, proliferation, differentiation, cell fate determination, apoptosis, signal transduction, host

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Use of Viral Systems to Study miRNA-Mediated Regulation of Gene Expression in Human Cells, viruses also encode miRNAs and these viral miRNAs target cellular and viral mRNAs to regulate virus replication and latent infection. In particular, herpesviruses encode a large number of miRNAs. Herpesvirus infection also changes the normal expression profile of cellular miRNAs. New genetic tools

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Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Celof endogenous small RNAs have advanced our knowledge in cancer progression and metastasis. Among many tools, small RNA cloning is a powerful method to identify new microRNAs (miRNAs) and to profile miRNA expression and function. Retroviral system is also the minimum requirement for the studying of m

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Preparing Synaptoneurosomes from Adult Mouse Forebrain, of synaptic membranes on sucrose or Percoll gradients. Alternatively, synaptoneurosomes are prepared by filtration of tissue homogenate through a series of filters to obtain a fraction that is enriched in pinched-off dendritic spines. Whereas the protocol for making synaptosomes is reasonably well

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