Titlebook: MicroRNA Profiling; Methods and Protocol Sweta Rani Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under exclusiv

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Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line,sults in subtle phenotype. Here we report a guideline on designing miRNA sponges inhibiting a miRNA family. As a model system, we targeted miR-30 family, known as tumor suppressor miRNAs in multiple tumors. To achieve an efficient knockdown, we generated perfect and bulged-matched miRNA binding site

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Assessment of Cell Cytotoxicity in 3D Biomaterial Scaffolds Following miRNA Transfection, effects within cells in both 2D and 3D microenvironments. The lactate dehydrogenase (LDH) assay is a colorimetric assay that provides a basic, dependable method for determining cellular cytotoxicity through assessment of the level of plasma membrane damage in a cell population. Here, we describe th

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Evaluation of miRNA Expression in 3D In Vitro Scaffold-Based Cancer Models,nt (TME) and accelerate the drug discovery pipeline. Previous studies examining the effects of specific miRNAs on cancer cells in vitro have involved ectopic expression of miRNA mimics in 2D in vitro culture. Assessment of cell viability and gene expression ensures that upregulation of the chosen mi

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Bioinformatics Analysis of miRNA Sequencing Data, identifying accurate microRNAs associated with the phenotypes of interest. A complete analysis process demands a high level of technical expertise in programming, statistics, and data management. The goal of this chapter is to reduce the burden of technical expertise and provide readers the opportu

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Plant MicroRNA Identification and Annotation Using Deep Sequencing Data,ave revealed that they play key roles in diverse life activities, such as growth and development. In the last decade, deep sequencing technology has generated substantial small RNA sequencing (sRNA-Seq) data. Meanwhile, numerous tools have been developed to identify miRNAs from these sRNA-Seq data,

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rowing political impetus worldwide for improved educational provision has stimulated countries’ willingness to participate in international large-scale comparative assessments of learning outcomes. However, research within the complex multinational context that IEA operates in has resulted in signif

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Neda Rahimian,Javid Sadri Nahand,Michael R. Hamblin,Hamed Mirzaei.Serves as a reference book.Enhancing the knowledge of the s.Defects generate a great economic problem for suppliers who are faced with increased duties. Customers expect increased efficiency and dependability of technical product of - also growing - complexity. The authors give an introduction to a

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Aisling Leavy,Eva M. Jimenez-Mateos.Serves as a reference book.Enhancing the knowledge of the s.Defects generate a great economic problem for suppliers who are faced with increased duties. Customers expect increased efficiency and dependability of technical product of - also growing - complexity. The authors give an introduction to a