| 書(shū)目名稱(chēng) | MicroRNA Profiling | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Sweta Rani | | 視頻video | http://file.papertrans.cn/633/632803/632803.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts | | 叢書(shū)名稱(chēng) | Methods in Molecular Biology | | 圖書(shū)封面 |  | | 描述 | .This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of .MicroRNA Profiling: Methods and Protocols?.discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and cutting-edge, .MicroRNA Profiling: Methods and Protocols. is a valuable resource for anyone interested in the field o | | 出版日期 | Book 2017 | | 關(guān)鍵詞 | human diseases; cell viability and proliferation; RNA isolation; formalin fixed paraffin embedded (FFPE | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-6524-3 | | isbn_softcover | 978-1-4939-8225-7 | | isbn_ebook | 978-1-4939-6524-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2017 |
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